The 19-year-old from Hawaii was competing for 30 spots at Woodmont Country Club, where most LPGA Tour players went the day after the LPGA Championship.
It will be the first major Wie has missed this year.
Wie has played in the U.S. Womens Open every year since she was 13. She had to qualify last year and then missed the cut at Interlachen.
Also failing to qualify were Natalie Gulbis and 18-year-old rookie Vicky Hurst.