Johnson, the two-time defending series champion, appeared to have the win at New Hampshire Motor Speedway locked up until a pair of late cautions gave Biffle a chance. Biffle trailed Johnson on a restart with 13 laps to go, but he passed Johnson one lap later and went on to snap a 33-race winless streak.
Johnson was second to jump two spots to the top of the Chase standings. Biffle, who came into the Chase opener in ninth, moved all the way to third in the points.
Kyle Busch had a horrible opener, finishing 35th because of an early broken sway bar and a later accident.