The 19-year-old from Hawaii was competing for one of 30 spots at Woodmont Country Club, where most LPGA Tour players went the day after the LPGA Championship.
It will be the first major Wie has missed this year.
Wie has played in the U.S. Women's Open every year since she was 13. She had to qualify last year, then missed the cut at Interlachen.
Also failing to qualify were Natalie Gulbis and 18-year-old rookie Vicky Hurst .
ALTERNATE'S LONG DAY: David Erdy , who gained entry to the U.S. Open as a first alternate after Japan's Shingo Katayama withdrew from competition with a back injury, narrowly averted disqualification Tuesday.
Official registration and check-in was supposed to conclude at 10 a.m. Tuesday, and though the USGA tries to be accommodating with travel issues and other problems, Erdy had not appeared nor made contact with officials by early afternoon.
Erdy, 19, was stuck in transit. He left Cincinnati at 6 a.m. and connected through Pittsburgh before landing at JFK Airport in New York. After getting lost a couple of times and arriving at the wrong hotel to pick up his credential, the Indiana resident finally made it to the registration room shortly after 4 p.m., before his spot was awarded to another alternate.
"They never made me feel like there was anything to worry about," he said.
AN EARLY LOOK: Anthony Kim played a full practice round at Bethpage Black last week and feels as though he knows where to go. He also caddied at the U.S. Open course last week and knows where not to go.
Kim took part in the Golf Digest Challenge , a made-for-TV event in which four amateurs try to break 100 on a U.S. Open course. He caddied for singer Justin Timberlake , who shot 88.
"Got to see some of the course that I probably don't want to see in the tournament," Kim said.
Kim has been an enigma this season after a breakthrough year in 2008, when he won at Quail Hollow and Congressional, and he gave the Americans a spark by whipping Sergio Garcia in the leadoff singles match at the Ryder Cup.
He opened the year with a runner-up finish at Kapalua. He hasn't had a top 10 since then.
Kim has dealt with a series of odd injuries, one to his jaw while horseback riding in New Zealand.
But there are concerns he is spending more time having fun off the course than putting in work on his game.
He has fallen to No. 15 in the world, and his best result since Kapalua was a tie for 20th in the Masters Tournament.
"To be honest, it's not far off," he said.
"I said that early last year before I went off and won a couple of tournaments. I just haven't been as patient as I need to be on the golf course, and if there's anywhere that's going to test it, it's going to be at Bethpage."
WHERE'S LEFTY? Phil Mickelson celebrated his 39th birthday Tuesday, but not on Bethpage Black.
Mickelson left the St. Jude Classic for his home in San Diego to be with his wife, Amy , who is battling breast cancer. He was to fly to New York later Tuesday, hold a news conference this morning and then play his lone practice round of the week.
Even so, the gallery was surprised to see his familiar sidekick -- caddie Jim "Bones" Mackay -- walking up the 13th fairway as he mapped out the course.