With the memory of Barbaro still fresh, Eight Belles' catastrophic breakdown Saturday put increasing focus on a sport already trying to overcome a decline in popularity.
Her death has raised thorny issues about the whole thoroughbred industry, including track safety, whether fillies should be allowed to run against colts, and whether horses are bred too much for speed and not for soundness.
A prominent animal rights group got involved Sunday, too, criticizing Eight Belles' jockey for whipping the horse and saying the second-place prize should be revoked.
But to horse people, it wasn't all that simple.
"To make it safer, don't race the horses, don't train them, then they'll live good lives out on the farm," Big Brown trainer Rick Dutrow Jr. said.
"But you have to train them for races, you have to run them and that's where the problems start to set in. They have to be asked to run and sometimes in a particular minute, they're asked to run when they're not ready to give it and then it hurts."
While Big Brown's bid to become the first Triple Crown winner in 30 years will certainly gain momentum in the next couple of weeks, Eight Belles and the sight of fans crying in the stands remained a focal point Sunday.
"Filly's Death Casts Shadow over Kentucky Derby," read The New York Times.
"Tragedy mars Kentucky Derby as the only filly dies after race," the Los Angeles Times' Web site said.
Churchill Downs officials were unsure whether there had been a fatality in the Kentucky Derby. Superintendent Butch Lehr said there hadn't been one in his 41 years at the track.
The death of Eight Belles may have been rare because it occurred well after the finish line, but it's just the latest trauma to happen at a major race on national television.
Two years ago, Derby winner Barbaro shattered his fight rear leg at the start of the Preakness, with more than 100,000 people gasping at the site of the undefeated colt in distress as he was led into an equine ambulance. Barbaro was euthanized eight months later after developing laminitis as a result of the injuries.
"It's difficult to accept, and we don't have all the answers," Scott Palmer, a veterinarian who helped attend to Barbaro on the track at Pimlico, said Sunday. "It's shocking to see something like that."
Now, there are more questions about track safety.
Barbaro's demise helped push forward the installation of synthetic surfaces to replace traditional dirt tracks at several tracks, including Keeneland, Santa Anita, Arlington Park, Hollywood Park, Golden Gate Fields, Del Mar, Turfway and Presque Isle. A new on-track injury reporting program seems to indicate the surface is having the desired effect.
Reports by veterinarians at 34 tracks across the country between June 2007 and early this year showed synthetic tracks averaged 1.47 fatalities per 1,000 starts, compared with 2.03 fatalities per 1,000 starts for horses that ran on dirt.
But not everyone is convinced.
"This is a very big issue and needs to be discussed," two-time Derby winning trainer Nick Zito said. "You're changing the whole game. Big Brown ran on dirt yesterday, he's going for history. You can't tell me the Polytrack is history. It's not yet, there isn't enough data yet."
That's not saying Zito and other horsemen are not interested in making racetracks safer for both horses and jockeys.
"If you told me, 'Look, we have a device that these horses can run on pillows and never get hurt the rest of lives,' I'd say, 'Where do I sign?'" Zito said. "There's injuries on the Polytrack, too. Now you see why I'm saying it's a big issue."
While breakdowns always have been a part of racing, there has been more of an outcry lately calling for drastic action.
People for the Ethical Treatment of Animals (PETA) issued a statement Sunday calling for the suspension of Eight Belles jockey Gabriel Saez. The group also asked for the "revocation of the second place prize."
Saez was riding in his first Kentucky Derby when Eight Belles broke both front ankles while galloping out a quarter-mile past the finish line.
"What we really want to know, did he feel anything along the way?" PETA spokeswoman Kathy Guillermo said. "If he didn't then we can probably blame the fact that they're allowed to whip the horses mercilessly."
A call to the jockeys' room at Delaware Park, where Saez raced on Sunday, went unanswered.
The Kentucky state racing stewards make decisions on suspensions, but there is no racing at Churchill Downs until Wednesday. At that time, the stewards could review a tape of the race if a formal request is made.
Eight Belles trainer Larry Jones disputed any suggestion that his horse had no business taking on the boys.
"It wasn't that, it wasn't the distance, it wasn't a big bumping match for her, she never got touched," he said. "She passed all those questions... with flying colors. The race was over, all we had to do was pull up, come back and be happy. It just didn't happen."
On Sunday morning, Jones stood next to his Kentucky Oaks-winning filly, Proud Spell, receiving condolences from friends and fellow trainers.
"Got here at 5 a.m.," Jones said. "Got to go on. It's hard, but it's what we do."
Just then, Barbaro's trainer Michael Matz drove past Jones' barn stopped his car and rolled down the window. On Friday, Matz watched another one of his horses, Chelokee, suffer a life-threatening injury in the Alysheba Stakes. He had just returned from Lexington, where the horse was set for surgery Monday to fuse his injured ankle.
"Sorry, Larry," Matz said.
"I know you know what it's like, thank you," Jones said. "How's yours doin'?"
"Doing good, they're going to operate tomorrow," Matz said.
Dutrow was still basking in Big Brown's victory, well aware that an injury can strike at any time.
"No matter what happens, you're always going to see horses break down on the track," he said. "That is part of this game. It's a very sad part of the game, but you have to go through it.
"For people coming out to the track and seeing that, it's got to make them think, 'Man, why would I want to go out there and see that happen to a horse?'" he said. "It's got to be very disappointing to anyone who loves horses."