Before the game, it was announced that Chipper Jones would be limited to pinch-hitting duty after he strained his right quadriceps in Monday night's game.
Then, in the 10th inning, catcher Brian McCann strained his left oblique, forcing him out of the game.
He'll be placed on the 15-day disabled list Wednesday.
McCann grabbed his left side and lower back in obvious pain after he threw high as Neil Walker stole second base in the 10th inning.
David Ross replaced McCann after trainer Jeff Porter came on the field to confer with McCann. McCann made a couple of test throws to pitcher Scott Linebrink before leaving the game.
The Braves have not announced a replacement for McCann on the roster.
Before the game, Jones said he is frustrated to be dealing with a new injury only one day after his return from the disabled list.
Jones, 39, had arthroscopic surgery on July 9 to repair torn cartilage in his right knee. He returned with two hits, including his ninth homer, Monday night.
He remained in the game after he strained his quadriceps on defense in the sixth inning and hit the homer in the eighth.
Braves manager Fredi Gonzalez said there is a 50-50 chance Jones could come in off the bench against the Pirates. Gonzalez says the team hasn't discussed placing Jones back on the disabled list.
The new injury concern had Jones in a bad mood.
"I'm tired of talking about things that are keeping me out of ballgames," Jones said. "It's getting old."
Jones said he felt a knot rising in the quadriceps area in Monday night's game and said the area was "very sore" on Tuesday. He said he expects to be available to pinch hit for a day or two before returning to the lineup. Jones did pinch hit Tuesday, grounding out to short to end the eighth.
Jones is hitting .262 with nine homers and 47 RBI in 78 games.
Martin Prado, the regular starter in left field who filled in at third base when Jones was on the disabled list, shifted back to third in Tuesday night's lineup.
Jordan Schafer was in the first lineup posted by Gonzalez before being scratched. Schafer jammed his left middle finger and has not started in six consecutive games.
Nate McLouth started in center field and Eric Hinske was added to the lineup in left field.