The Buckeyes moved up one spot in the Top 25 after preseason No. 1 Alabama lost for the first time this season. The Crimson Tide fell 35-21 on Saturday at South Carolina.
Oregon is No. 2, followed by Boise State, TCU, Nebraska, Oklahoma and Auburn.
Ohio State received 34 first-place votes and Oregon (15), Boise State (eight), TCU (one) and Oklahoma (two) also got first-place votes from the media panel.
The Crimson Tide slipped to No. 8, LSU was No. 9 and South Carolina moved up nine spots to 10th after the program's first victory against a top-ranked team.
The last time Ohio State was No. 1 was the final regular season poll of 2007.