The announcement came a day after she informed the school system's central office of her intention, Superintendent Charles Nagle said.
Though Hamilton no longer will be principal, Nagle said he told her she can remain with the school system in another capacity if she chooses.
Several of Hamilton's educators targeted her in a September letter sent to her, Nagle and school board Chairwoman Regina Buccafusco. The letter accused the principal of poor leadership and bullying teachers.
Signed by 14 staff members at Greenbrier High, the letter said Hamilton is "harsh, commanding and intimidating." The authors also contended that Hamilton created an atmosphere of "constant fear that permeates throughout the staff."
A cover letter sent to Nagle said about 28 percent of the school's staff were not asked to sign the letter because they were new and that many other teachers refused to sign for fear of losing their jobs.
However, it isn't clear whether the chasm between Hamilton and teachers is the reason behind her decision to leave the school.
A phone message left with Hamilton's secretary on Thursday, after school hours, was not immediately returned.
Nagle said Hamilton chose to quit for "personal reasons."
Hamilton's resignation will be discussed by the school board during a Tuesday meeting, Nagle said. Personnel matters typically are discussed in executive session.
Reach Donnie Fetter at (706) 868-1222, ext. 115,