Police say 13-year-old Justin Milledge and 14-year-old Marquis Milledge were unresponsive Sunday.
Guests at the Regency Inn Hotel on East Oglethorpe Boulevard pulled the boys from the water and performed CPR on them. Authorities say a sister was with the boys but it's unclear if she was supposed to be supervising them.
It happened around 5:30 p.m. The brothers were later air lifted to an Augusta hospital.
Authorities have recorded two drownings in Albany this summer.