Anshonoe Mills claimed in a lawsuit filed Wednesday that Holmes grabbed her face and threw the glass at her because she was sitting on a couch in the Rain Night Club's VIP section on March 7.
The lawsuit said the glass hit Mills on the right side of the face, cutting the woman near the eye. The four-page document also claimed Holmes and a police officer intimidated Mills so she wouldn't press charges.
Holmes offered to pay Mills because he was an NFL star and couldn't afford to get into trouble, the lawsuit said. The woman felt pressured and incoherently told police that she was hit in the face and was bleeding but wouldn't press charges, according to the lawsuit.
Holmes' teammate, quarterback Ben Roethlisberger, was accused earlier this month of assaulting a 20-year-old Georgia college student. He has not been charged in that case.
Mills is seeking more than $15,000 in damages from Holmes, who was MVP of the Super Bowl played after the 2008 season.
Steelers spokesman Dave Lockett said team officials were gathering information and had no further comment.